Anticorps Monoclonal anti-ARNT,HIF1B

ARNT,HIF1B Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

3B12C5

N° de cat : 66732-1-Ig

Synonymes

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

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Galerie de données de validation

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  WB analysis using 66732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66732-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66732-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66732-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 66732-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules NIH/3T3
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66732-1-Ig cible ARNT,HIF1B dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARNT,HIF1B Protéine recombinante Ag5507
• Nom complet: aryl hydrocarbon receptor nuclear translocator
• Masse moléculaire calculée: 416 aa, 46 kDa, 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC060838
• Symbole du gène: ARNT
• Identification du gène (NCBI): 405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia