Anticorps Recombinant de lapin anti-ARNTL

• Phare
• Validé par KD/KO

ARNTL Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250536E10

N° de cat : 86113-1-PBS

Synonymes

BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

---

Galerie de données de validation

• 

  WB analysis using 86113-1-RR (same clone as 86113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86113-1-RR (ARNTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  WB result of ARNTL antibody (86113-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNTL transfected HeLa cells. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARNTL antibody (86113-1-RR, Clone: 250536E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

86113-1-PBS cible ARNTL dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARNTL Protéine recombinante Ag5586
• Nom complet: aryl hydrocarbon receptor nuclear translocator-like
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 69-75 kDa
• Numéro d’acquisition GenBank: BC041129
• Symbole du gène: ARNTL
• Identification du gène (NCBI): 406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

{{ptg:RelatedPrimaryAntibodies}}