Anticorps Polyclonal de lapin anti-ARP3/ARP3B

• Phare
• Validé par KD/KO

ARP3/ARP3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13822-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13822-1-AP (same clone as 13822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 13822-1-AP (same clone as 13822-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

  IP result of anti-ARP3/ARP3B (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13822-1-AP (same clone as 13822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13822-1-PBS cible ARP3/ARP3B dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARP3/ARP3B Protéine recombinante Ag4922
• Nom complet: ARP3 actin-related protein 3 homolog (yeast)
• Masse moléculaire calculée: 418 aa 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC044590
• Symbole du gène: ARP3/ACTR3
• Identification du gène (NCBI): 10096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

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