Anticorps Monoclonal anti-ARP3/ARP3B

ARP3/ARP3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D12C7

N° de cat : 68439-1-PBS

Synonymes

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

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Galerie de données de validation

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  WB analysis using 68439-1-Ig (same clone as 68439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68439-1-PBS cible ARP3/ARP3B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARP3/ARP3B Protéine recombinante Ag5017
• Nom complet: ARP3 actin-related protein 3 homolog (yeast)
• Masse moléculaire calculée: 418 aa 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC044590
• Symbole du gène: ARP3/ACTR3
• Identification du gène (NCBI): 10096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

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