Anticorps Polyclonal de lapin anti-ARPC2

• Phare
• Validé par KD/KO

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15058-1-PBS

Synonymes

Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC

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Galerie de données de validation

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  WB analysis using 15058-1-AP (same clone as 15058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15058-1-AP (same clone as 15058-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15058-1-AP (same clone as 15058-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15058-1-AP (same clone as 15058-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 15058-1-AP (same clone as 15058-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of 3T3-L1 using 15058-1-AP (same clone as 15058-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 15058-1-AP (same clone as 15058-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 15058-1-AP (same clone as 15058-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  IP result of anti-ARPC2 (IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15058-1-AP (same clone as 15058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15058-1-AP (same clone as 15058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15058-1-PBS cible ARPC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPC2 Protéine recombinante Ag7041
• Nom complet: actin related protein 2/3 complex, subunit 2, 34kDa
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000590
• Symbole du gène: ARPC2
• Identification du gène (NCBI): 10109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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