Anticorps Monoclonal anti-ARPC2

• Phare
• Validé par KD/KO

ARPC2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D8E3

N° de cat : 68145-1-PBS

Synonymes

1D8E3, Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC

---

Galerie de données de validation

• 

  WB analysis using 68145-1-Ig (same clone as 68145-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  WB result of ARPC2 antibody (68145-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARPC2 transfected HeLa cells. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68145-1-PBS cible ARPC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARPC2 Protéine recombinante Ag7082
• Nom complet: actin related protein 2/3 complex, subunit 2, 34kDa
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000590
• Symbole du gène: ARPC2
• Identification du gène (NCBI): 10109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}