Anticorps Polyclonal de lapin anti-ARPP-19

• Phare
• Validé par KD/KO

ARPP-19 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11678-1-AP

Synonymes

ARPP 16, ARPP 19, ARPP16, ARPP19, ARPP-19

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Galerie de données de validation

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  WB analysis of Jurkat using 11678-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11678-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11678-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11678-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 11678-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11678-1-AP (ARPP-19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11678-1-AP

  IP Result of anti-ARPP-19 (IP:11678-1-AP, 3ug; Detection:11678-1-AP 1:500) with mouse brain tissue lysate 5200ug.

• 

  IHC staining of human gliomas using 11678-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11678-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11678-1-AP (ARPP-19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 11678-1-AP

  Immunofluorescent analysis of A375 cells using 11678-1-AP (ARPP-19 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375, cellules A549, tissu cérébral de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11678-1-AP cible ARPP-19 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPP-19 Protéine recombinante Ag2279
• Nom complet: cyclic AMP phosphoprotein, 19 kD
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC003418
• Symbole du gène: ARPP-19
• Identification du gène (NCBI): 10776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARPP-19, a 19-kDa cAMP-regulated phosphoprotein, plays a role in regulating mitosis. Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A). When phosphorylated by protein kinase Greatwall (Gwl), ARPP-19 associate with and inhibit PP2A, thus promoting mitotic entry. ARPP-19 may be an important link between nerve growth factor (NGF) signaling and post-transcriptional control of neuronal gene expression such as GAP-43.

Publications

Species	Application	Title
human	WB	Front Oncol; Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.; Authors - Xigang Xia
human	WB	J Proteome Res; Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression.; Authors - Luqi Jin
human	WB,IHC	Onco Targets Ther; PMID: 32753897; Authors - Xiang Gao ; KD Validated
human	WB	Int J Mol Sci; Increased ARPP-19 expression is associated with hepatocellular carcinoma.; Authors - Haiyan Song
human	WB	Cancers (Basel); Arpp19 Promotes Myc and Cip2a Expression and Associates with Patient Relapse in Acute Myeloid Leukemia.; Authors - Eleonora Mäkelä ; KD Validated
human	WB,IP	Nature; Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19; Authors - Sathish K R Padi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

ARPP19 antibody giving a very clear sharp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer cells