Anticorps Polyclonal de lapin anti-Beta Arrestin 1

• Phare
• Validé par KD/KO

Beta Arrestin 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 15361-1-AP

Synonymes

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1, β-Arrestin-1

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Galerie de données de validation

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  WB analysis using 15361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15361-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15361-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 15361-1-AP

  IP Result of anti-beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules RAW 264.7, cellules U-937, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cérébral de souris, tissu de cervelet de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15361-1-AP cible Beta Arrestin 1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta Arrestin 1 Protéine recombinante Ag7608
• Nom complet: arrestin, beta 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47-55 kDa
• Numéro d’acquisition GenBank: BC003636
• Symbole du gène: ARRB1
• Identification du gène (NCBI): 408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Metab; Autonomous sensing of the insulin peptide by an olfactory G protein-coupled receptor modulates glucose metabolism.; Authors - Jie Cheng ; KD Validated
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
	IF	Cell Death Dis; β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.; Authors - J Liu
human	WB	J Biol Chem; An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.; Authors - Jing-Yu Lin ; KD Validated
human	WB	PLoS One; Cell Survival Following Radiation Exposure Requires miR-525-3p Mediated Suppression of ARRB1 and TXN1.; Authors - Kraemer Anne A
mouse	IF	Oncol Rep; Blue light irradiation inhibits the growth of colon cancer and activation of cancer‑associated fibroblasts.; Authors - Toshiaki Yoshimoto