Anticorps Polyclonal de lapin anti-Beta Arrestin 2

• Phare
• Validé par KD/KO

Beta Arrestin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 10171-1-AP

Synonymes

ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2, β-Arrestin 2

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Galerie de données de validation

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  WB analysis using 10171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10171-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10171-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10171-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsil using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10171-1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 10171-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10171-1-AP (Beta Arrestin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10171-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Arrestin 2 antibody (10171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of mouse Retina tissue using 10171-1-AP

  IF result of anti-ARRB2 (10171-1-AP) in Mouse Retina by Dr. Seongjin Seo. IS (Inner segment) Stain.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules PC-12, RAW264.7, tissu cérébral de rat
• Résultats positifs en IHC: tissu amygdalien humain, tissu de cancer du côlon humain, tissu de mélanome malin humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, tissu rétinien de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10171-1-AP cible Beta Arrestin 2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta Arrestin 2 Protéine recombinante Ag0227
• Nom complet: arrestin, beta 2
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC007427
• Symbole du gène: ARRB2
• Identification du gène (NCBI): 409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.; Authors - Yuqing Wu ; KO Validated
rat	WB	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
human,mouse	WB,IF,CoIP	J Pharm Anal; Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis; Authors - Jia-Jia Zhang ; KD Validated
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
mouse	IF	Acta Pharmacol Sin; Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy.; Authors - Tian-Tian Wei
human	WB	Development; The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.; Authors - Verena Rauschenberger

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