Anticorps Polyclonal de lapin anti-ASAH1

ASAH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 11274-1-AP

Synonymes

AC, Acid ceramidase, Acid ceramidase subunit alpha, Acid ceramidase subunit beta, ASAH

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Galerie de données de validation

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  WB analysis using 11274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 11274-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASAH1 antibody (11274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, cellules MDA-MB-231, cellules SH-SY5Y, cellules SW480, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11274-1-AP cible ASAH1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASAH1 Protéine recombinante Ag1799
• Nom complet: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC016828
• Symbole du gène: ASAH1
• Identification du gène (NCBI): 427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.; Authors - Judith Lang
mouse,human	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
human	WB	Hum Mol Genet; Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.; Authors - Myung Jong Kim
mouse	IF	BMC Genomics; Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment; Authors - Yuanmin Jia
mouse	WB	Int J Mol Sci; Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.; Authors - Yu-Ting Su
human	WB	J Dermatol Sci; Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.; Authors - Sawada Eri E

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