Anticorps Polyclonal de lapin anti-ASBT

ASBT Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 25245-1-AP

Synonymes

ASBT, IBAT, ISBT, NTCP2, SLC10A2

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Galerie de données de validation

• 

  WB analysis of mouse small intestine using 25245-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25245-1-AP (ASBT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 25245-1-AP

  IP Result of anti-ASBT (IP:25245-1-AP, 4ug; Detection:25245-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris,
• Résultats positifs en IP: tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below

Informations sur le produit

25245-1-AP cible ASBT dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASBT Protéine recombinante Ag18808
• Nom complet: solute carrier family 10 (sodium/bile acid cotransporter family), member 2
• Masse moléculaire calculée: 348 aa, 38 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC130523
• Symbole du gène: SLC10A2
• Identification du gène (NCBI): 6555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu
mouse	WB	Food Funct; Apple polyphenol extract modulates bile acid metabolism and gut microbiota by regulating the circadian rhythms in daytime-restricted high fat diet feeding C57BL/6 male mice.; Authors - Yuan Cui
mouse	WB	Biochim Biophys Acta; Rhizoma Coptidis alkaloids alleviate hyperlipidemia in B6 mice by modulating gut microbiota and bile acid pathways.; Authors - Kai He
	WB	Biochim Biophys Acta Biomembr; S-acylation status of bile acid transporter hASBT regulates its function, metabolic stability, membrane expression, and phosphorylation state.; Authors - Ebehiremen N Ayewoh

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

It does not work as expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver, mouse small intestine, mouse kidney, HepG2

ASBT Antibody Western Blot validation (1:500 dilution) in Mouse liver, mouse small intestine, mouse kidney, HepG2 (Cat no:25245-1-AP)