Anticorps Polyclonal de lapin anti-SLC10A2/ASBT

SLC10A2/ASBT Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 25245-1-AP

Synonymes

ASBT, SLC10A2, Apical sodium-dependent bile acid transporter, Na(+)-dependent ileal bile acid transporter, ISBT

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Galerie de données de validation

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  WB analysis of mouse small intestine using 25245-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25245-1-AP (ASBT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 25245-1-AP

  IP result of anti-ASBT (IP:25245-1-AP, 4ug; Detection:25245-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris,
• Résultats positifs en IP: tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

25245-1-AP cible SLC10A2/ASBT dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC10A2/ASBT Protéine recombinante Ag18808
• Nom complet: solute carrier family 10 (sodium/bile acid cotransporter family), member 2
• Masse moléculaire calculée: 348 aa, 38 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC130523
• Symbole du gène: ASBT
• Identification du gène (NCBI): 6555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC10A2 also known as ASBT, ISBT, and NTCP2, is a sodium/bile acid cotransporter that is primarily responsible for the uptake of bile acids by apical cells in the distal ileum(PMID: 8661017). Mutations in SLC10A2 cause primary bile acid malabsorption (PBAM) and may be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG)(PMID: 9109432).

Publications

Species	Application	Title
mouse	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
human,mouse	WB,IHC	Cell Host Microbe; Veillonella intestinal colonization promotes C. difficile infection in Crohn's disease; Authors - Ziyu Yang
mouse	WB	Nat Commun; Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice; Authors - Xiaoxue Jiang
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

It does not work as expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver, mouse small intestine, mouse kidney, HepG2

SLC10A2/ASBT Antibody Western Blot validation (1:500 dilution) in Mouse liver, mouse small intestine, mouse kidney, HepG2 (Cat no:25245-1-AP)

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