Anticorps Polyclonal de lapin anti-ASC/TMS1

• Phare
• Validé par KD/KO

ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10500-1-PBS

Synonymes

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC

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Galerie de données de validation

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  WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 10500-1-AP (same clone as 10500-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10500-1-AP (same clone as 10500-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 10500-1-AP (same clone as 10500-1-PBS)

  IP result of anti-ASC/TMS1 (IP:10500-1-AP, 4ug; Detection:10500-1-AP 1:10000) with HL-60 cells lysate 2240 ug. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (TMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10500-1-AP (same clone as 10500-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10500-1-AP (TMS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10500-1-PBS cible ASC/TMS1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASC/TMS1 Protéine recombinante Ag0774
• Nom complet: PYD and CARD domain containing
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC004470
• Symbole du gène: ASC/TMS1
• Identification du gène (NCBI): 29108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

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