Anticorps Monoclonal anti-ASC/TMS1

ASC/TMS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(37)

CloneNo.

2G12E6

N° de cat : 67494-1-Ig

Synonymes

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

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Galerie de données de validation

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  WB analysis using 67494-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human placenta using 67494-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67494-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67494-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67494-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of MCF-7 using 67494-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of THP-1 using 67494-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules THP-1, cellules U-937, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

67494-1-Ig cible ASC/TMS1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASC/TMS1 Protéine recombinante Ag28424
• Nom complet: PYD and CARD domain containing
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC004470
• Symbole du gène: ASC/TMS1
• Identification du gène (NCBI): 29108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Publications

Species	Application	Title
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
	WB,IP,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
	WB,IF	Sci Adv; Asparagine transporter supports macrophage inflammation via histone phosphorylation; Authors - Chuanlong Wang
	WB	Oxid Med Cell Longev; PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.; Authors - Zeyu Li
	WB	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
	WB,IF	J Virol; Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice; Authors - Qiongqiong Zhou

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