Anticorps Monoclonal anti-ASC/TMS1

ASC/TMS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2G12E6

N° de cat : 67494-1-PBS

Synonymes

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

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Galerie de données de validation

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  WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67494-1-Ig (same clone as 67494-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67494-1-PBS cible ASC/TMS1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASC/TMS1 Protéine recombinante Ag28424
• Nom complet: PYD and CARD domain containing
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC004470
• Symbole du gène: ASC/TMS1
• Identification du gène (NCBI): 29108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

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