Anticorps Polyclonal de lapin anti-ASCC2

ASCC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11529-1-PBS

Synonymes

ASC-1 complex subunit p100, ASC1P100, RQT3

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Galerie de données de validation

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  WB analysis using 11529-1-AP (same clone as 11529-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11529-1-AP (same clone as 11529-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 11529-1-AP (same clone as 11529-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 11529-1-AP (same clone as 11529-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11529-1-AP (same clone as 11529-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11529-1-AP (same clone as 11529-1-PBS)

  IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11529-1-AP (same clone as 11529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11529-1-PBS cible ASCC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASCC2 Protéine recombinante Ag2100
• Nom complet: activating signal cointegrator 1 complex subunit 2
• Masse moléculaire calculée: 757 aa, 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC025368
• Symbole du gène: ASCC2
• Identification du gène (NCBI): 84164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).

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