Anticorps Recombinant de lapin anti-ASCC2

ASCC2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250566D5

N° de cat : 86086-1-PBS

Synonymes

ASC-1 complex subunit p100, ASC1P100, RQT3

---

Galerie de données de validation

• 

  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86086-1-RR against Human ASCC2 were performed. The affinity constant is 26.5 pM.

---

Informations sur le produit

86086-1-PBS cible ASCC2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASCC2 Protéine recombinante Ag2100
• Nom complet: activating signal cointegrator 1 complex subunit 2
• Masse moléculaire calculée: 757 aa, 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC025368
• Symbole du gène: ASCC2
• Identification du gène (NCBI): 84164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).

{{ptg:RelatedPrimaryAntibodies}}