Anticorps Recombinant de lapin anti-ASCC3

ASCC3 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242755B4

N° de cat : 85130-2-PBS

Synonymes

242755B4, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, ASC1p200, EC:3.6.4.12

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Galerie de données de validation

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  WB analysis using 85130-2-RR (same clone as 85130-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85130-2-RR (ASCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85130-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01828-3

  Sandwich ELISA standard curve of MP01828-3, Human ASCC3 Recombinant Matched Antibody Pair - PBS only. 85130-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11848. 85130-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

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  Sample test of MP01828-3

  The mean ASCC3 concentration was determined to be 17.15 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 23.98 ng/mL in HepG2 cell extract based on a 1.80 mg/mL extract load.

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  IF Staining of HeLa using 85130-2-RR (same clone as 85130-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASCC3 antibody (85130-2-RR, Clone: 242755B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85130-2-PBS in a different storage buffer formulation.

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Informations sur le produit

85130-2-PBS cible ASCC3 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASCC3 Protéine recombinante Ag11848
• Nom complet: activating signal cointegrator 1 complex subunit 3
• Masse moléculaire calculée: 111 aa, 13 kDa, 251 kDa
• Poids moléculaire observé: 245 kDa
• Numéro d’acquisition GenBank: BC050681
• Symbole du gène: ASCC3
• Identification du gène (NCBI): 10973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.

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