Anticorps Monoclonal anti-ASF1A

ASF1A Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D4A6

N° de cat : 66884-1-PBS

Synonymes

2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98

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Galerie de données de validation

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  WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66884-1-Ig (same clone as 66884-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1A antibody (66884-1-Ig, Clone: 2D4A6 ) at dilution of 1:500 and sms3AF647-1, CL488-Phalloidin (green). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51470-1

  Cytometric bead array sample test of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS.

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  Cytometric bead array standard curve of MP51470-1

  Cytometric bead array standard curve of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS. Standard:Ag1233. Range: 0.781-100 ng/mL.

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Informations sur le produit

66884-1-PBS cible ASF1A dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASF1A Protéine recombinante Ag1233
• Nom complet: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 204 aa, 23 kDa
• Poids moléculaire observé: 21-23 kDa
• Numéro d’acquisition GenBank: BC010878
• Symbole du gène: ASF1A
• Identification du gène (NCBI): 25842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

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