Anticorps Recombinant de lapin anti-ASF1B

ASF1B Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250195A7

N° de cat : 85848-1-PBS

Synonymes

ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II

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Galerie de données de validation

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  WB analysis using 85848-1-RR (same clone as 85848-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85848-1-RR (ASF1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85848-1-RR (same clone as 85848-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1B antibody (85848-1-RR, Clone: 250195A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02155-2

  Cytometric bead array standard curve of MP02155-2, ASF1B-specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85848-3-PBS. Detection antibody: 85848-1-PBS. Standard: Ag17763. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 85848-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85848-1-RR against Human ASF1B were performed. The affinity constant is 57.7 nM.

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Informations sur le produit

85848-1-PBS cible ASF1B dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASF1B Protéine recombinante Ag17763
• Nom complet: ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 202 aa, 22 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC010014
• Symbole du gène: ASF1B
• Identification du gène (NCBI): 55723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.

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