Anticorps Polyclonal de lapin anti-ASGR1

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31338-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 31338-1-AP (same clone as 31338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31338-1-AP (ASGR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31338-1-PBS cible ASGR1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: asialoglycoprotein receptor 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: NM_001671.4
• Symbole du gène: ASGR1
• Identification du gène (NCBI): 432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

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