Anticorps Monoclonal anti-ASGR1

• Phare
• Validé par KD/KO

ASGR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5F5

N° de cat : 66692-1-Ig

Synonymes

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 66692-1-Ig

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells.

• 

  WB analysis using 66692-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66692-1-Ig

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells.

• 

  WB analysis of pig liver using 66692-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66692-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66692-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse hepatocytes using 66692-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66692-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66692-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 66692-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules HepG2, cellules HSC-T6, cellules L02, hépatocytes de souris, tissu hépatique de porc
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66692-1-Ig cible ASGR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASGR1 Protéine recombinante Ag2310
• Nom complet: asialoglycoprotein receptor 1
• Masse moléculaire calculée: 291 aa, 33 kDa
• Poids moléculaire observé: 42-46 kDa
• Numéro d’acquisition GenBank: BC032130
• Symbole du gène: ASGR1
• Identification du gène (NCBI): 432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.