Anticorps Recombinant de lapin anti-ASGR1

ASGR1 Recombinant Antibody for WB, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240672C4

N° de cat : 83691-3-PBS

Synonymes

asialoglycoprotein receptor 1, HL-1, 240672C4, ASGP R 1, ASGPR

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Galerie de données de validation

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  WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA Sample test of MP00681-3

  The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

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  Sandwich ELISA Sample test of MP00681-3

  Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

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  Sandwich ELISA standard curve of MP00681-3

  Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

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  IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00681-3

  Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83691-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

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Informations sur le produit

83691-3-PBS cible ASGR1 dans les applications de WB, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASGR1 Protéine recombinante Eg0946
• Nom complet: asialoglycoprotein receptor 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 42-46 kDa
• Numéro d’acquisition GenBank: NM_001671.4
• Symbole du gène: ASGR1
• Identification du gène (NCBI): 432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

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