Anticorps Polyclonal de lapin anti-ASGR2

ASGR2 Polyclonal Antibody for WB, IHC, FC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11501-2-PBS

Synonymes

CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2

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Galerie de données de validation

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  WB analysis of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 11501-2-AP (same clone as 11501-2-PBS)

  IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11501-2-AP (same clone as 11501-2-PBS)

  1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11501-2-PBS cible ASGR2 dans les applications de WB, IHC, FC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASGR2 Protéine recombinante Ag2037
• Nom complet: asialoglycoprotein receptor 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC017251
• Symbole du gène: ASGR2
• Identification du gène (NCBI): 433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

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