Anticorps Monoclonal anti-ASH2L

ASH2L Monoclonal Antibody for Indirect ELISA, IHC, WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

Indirect ELISA, IHC, WB

Conjugaison

Non conjugué

CloneNo.

1G1H3

N° de cat : 67859-1-PBS

Synonymes

Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein

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Galerie de données de validation

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  WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67859-1-Ig (same clone as 67859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67859-1-Ig (same clone as 67859-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67859-1-PBS cible ASH2L dans les applications de Indirect ELISA, IHC, WB et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASH2L Protéine recombinante Ag3008
• Nom complet: ash2 (absent, small, or homeotic)-like (Drosophila)
• Masse moléculaire calculée: 628 aa, 69 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC015936
• Symbole du gène: ASH2L
• Identification du gène (NCBI): 9070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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