Anticorps Monoclonal anti-ASK1

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C3A10

N° de cat : 67072-1-PBS

Synonymes

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

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Galerie de données de validation

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  WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67072-1-Ig (same clone as 67072-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67072-1-Ig (same clone as 67072-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67072-1-Ig (same clone as 67072-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67072-1-Ig (same clone as 67072-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 67072-1-Ig (same clone as 67072-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67072-1-PBS cible ASK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASK1 Protéine recombinante Ag5818
• Nom complet: mitogen-activated protein kinase kinase kinase 5
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC054503
• Symbole du gène: ASK1
• Identification du gène (NCBI): 4217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

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