Anticorps Monoclonal anti-ASNA1

ASNA1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, poulet, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B7C11

N° de cat : 66346-1-PBS

Synonymes

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

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Galerie de données de validation

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  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66346-1-Ig (same clone as 66346-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66346-1-Ig (same clone as 66346-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66346-1-Ig (same clone as 66346-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50801-1

  Cytometric bead array standard curve of MP50801-1, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-2-PBS. Standard:Ag7936. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50801-2

  Cytometric bead array standard curve of MP50801-2, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-3-PBS. Standard:Ag7936. Range: 0.391-100 ng/mL.

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Informations sur le produit

66346-1-PBS cible ASNA1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, poulet, rat, souris

• Réactivité: Humain, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASNA1 Protéine recombinante Ag7936
• Nom complet: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Masse moléculaire calculée: 38.7 kDa
• Poids moléculaire observé: 37-41 kDa
• Numéro d’acquisition GenBank: BC002651
• Symbole du gène: ASNA1
• Identification du gène (NCBI): 439
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

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