Anticorps Polyclonal de lapin anti-ASNS

• Phare
• Validé par KD/KO

ASNS Polyclonal Antibody for IF, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (2)

N° de cat : 14681-1-AP

Synonymes

ASNS, asparagine synthetase, TS11

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Galerie de données de validation

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  WB analysis of Jurkat using 14681-1-AP

  WB result of ASNS antibody (14681-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASNS transfected Jurkat cells.

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  WB analysis using 14681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14681-1-AP (ASNS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14681-1-AP

  IP Result of anti-ASNS (IP:14681-1-AP, 4ug; Detection:14681-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human retinoblastoma using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 14681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14681-1-AP (ASNS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de rétinoblastome humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 38 publications below
• IHC: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

14681-1-AP cible ASNS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASNS Protéine recombinante Ag6393
• Nom complet: asparagine synthetase
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC014621
• Symbole du gène: ASNS
• Identification du gène (NCBI): 440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.; Authors - Robert T Williams
human	WB	Cell Metab; Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.; Authors - Abigail S Krall ; KO Validated
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB, IHC	Mol Cell; Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.; Authors - Ji Zhang ; KD Validated
human	WB	Nat Commun; p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.; Authors - Longfei Deng ; KD Validated
human	IHC	J Exp Med; Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.; Authors - Maria Victoria Recouvreux

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (12-02-2019)

Human RCC4 kidney cancer cell lysate was denatured in SDS-sample buffer and size-separated on a denaturing gel.A specific band appeared for ASNS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

ASNS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:14681-1-AP)

FH

Daniel (Verified Customer) (12-02-2019)

Mouse kidney stained for Asns (green, AF488) and nucleus (blue, DAPI).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

ASNS Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:14681-1-AP)

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