Anticorps Polyclonal de lapin anti-ASPN

ASPN Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27602-1-PBS

Synonymes

SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1

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Galerie de données de validation

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  WB analysis of HepG2 using 27602-1-AP (same clone as 27602-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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  WB analysis using 27602-1-AP (same clone as 27602-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 27602-1-AP (same clone as 27602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27602-1-AP (ASPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27602-1-PBS cible ASPN dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASPN Protéine recombinante Ag26380
• Nom complet: asporin
• Masse moléculaire calculée: 384 aa, 44 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC063114
• Symbole du gène: ASPN
• Identification du gène (NCBI): 54829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASPN, also known as periodontal ligament-associated protein1 (PLAP1), is an extracellular matrix (ECM) protein. The calculated molecular weight of ASPN is 43 kDa. It belongs to the small leucine-rich proteoglycans (SLRPs) family and contains a unique aspartate-rich N terminus that distinguishes it from other SLRPs family members. It was firstly identified in human cartilage and it was associated with the pathogenesis of bone and joint diseases, including osteoarthritis, intervertebral disc degeneration and periodontal ligament mineralization (PMID:11152692; 26089687).

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