Anticorps Monoclonal anti-ASS1

• Phare
• Validé par KD/KO

ASS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1A11

N° de cat : 66036-1-PBS

Synonymes

1D1A11, ASS, EC:6.3.4.5

---

Galerie de données de validation

• 

  WB analysis of pig liver using 66036-1-Ig (same clone as 66036-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66036-1-Ig (same clone as 66036-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66036-1-Ig (same clone as 66036-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66036-1-PBS cible ASS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASS1 Protéine recombinante Ag9314
• Nom complet: argininosuccinate synthetase 1
• Masse moléculaire calculée: 412 aa, 47 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC009243
• Symbole du gène: ASS1
• Identification du gène (NCBI): 445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

{{ptg:RelatedPrimaryAntibodies}}