Anticorps Polyclonal de lapin anti-ASZ1

ASZ1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 21550-1-AP

Synonymes

ALP1, ANKL1, Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1, Ankyrin-like protein 1, ASZ 1

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Galerie de données de validation

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  WB analysis of mouse testis using 21550-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21550-1-AP (ASZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21550-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21550-1-AP (ASZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21550-1-AP

  IP result of anti-ASZ1 (IP:21550-1-AP, 4ug; Detection:21550-1-AP 1:600) with mouse testis tissue lysate 1560 ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

21550-1-AP cible ASZ1 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASZ1 Protéine recombinante Ag16217
• Nom complet: ankyrin repeat, SAM and basic leucine zipper domain containing 1
• Masse moléculaire calculée: 475 aa, 53 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC034963
• Symbole du gène: ASZ1
• Identification du gène (NCBI): 136991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF, WB	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding
mouse	IF	Dev Cell; TDRD1 phase separation drives intermitochondrial cement assembly to promote piRNA biogenesis and fertility; Authors - Jie Gao
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang
mouse	WB	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu
mouse	IF	Nucleic Acids Res; Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.; Authors - Deqiang Ding

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