Anticorps Polyclonal de lapin anti-ATF1

• Phare
• Validé par KD/KO

ATF1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11946-1-PBS

Synonymes

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

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Galerie de données de validation

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  WB analysis of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11946-1-AP (same clone as 11946-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11946-1-AP (same clone as 11946-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (11946-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11946-1-PBS cible ATF1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATF1 Protéine recombinante Ag2548
• Nom complet: activating transcription factor 1
• Masse moléculaire calculée: 271 aa, 29 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC029619
• Symbole du gène: ATF1
• Identification du gène (NCBI): 466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

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