Anticorps Polyclonal de lapin anti-ATF4

ATF4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 28657-1-AP

Synonymes

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

---

Galerie de données de validation

• 

  WB analysis using 28657-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28657-1-AP (ATF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 28657-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28657-1-AP (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

28657-1-AP cible ATF4 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATF4 Protéine recombinante Ag29844
• Nom complet: activating transcription factor 4 (tax-responsive enhancer element B67)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022088
• Symbole du gène: ATF4
• Identification du gène (NCBI): 468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Publications

Species	Application	Title
mouse	WB	Autophagy; Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.; Authors - Wenwen Liu
mouse	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
human	WB	Inflammation; CCAAT/Enhancer-Binding Protein Homologous Protein (CHOP) Deficiency Attenuates Heatstroke-Induced Intestinal Injury; Authors - Yan Cao
human,rat	WB	Int J Ophthalmol; SIRT1 inhibits apoptosis of human lens epithelial cells through suppressing endoplasmic reticulum stress in vitro and in vivo; Authors - Hui Cui
human	WB	bioRxiv; The Integrated Stress Response Suppresses PINK1-dependent Mitophagy by Preserving Mitochondrial Import Efficiency; Authors - Mingchong Yang
mouse	WB	Int J Mol Sci; Epalrestat Alleviates Reactive Oxygen Species and Endoplasmic Reticulum Stress by Maintaining Glycosylation in IMS32 Schwann Cells Under Exposure to Galactosemic Conditions; Authors - Hideji Yako

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Danyan (Verified Customer) (09-29-2025)

the antibody showed strong, specific binding in my tissue samples with little to no non-specific signal.

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Cell Tissue Type: HEK293T

{{ptg:RelatedPrimaryAntibodies}}