Anticorps Recombinant de lapin anti-ATF4

ATF4 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

230306B5

N° de cat : 81798-2-RR

Synonymes

230306B5, cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67

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Galerie de données de validation

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  WB analysis using 81798-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81798-2-RR (ATF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 81798-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (81798-2-RR, Clone: 230306B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HEK-293 using 81798-2-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug ATF4 Recombinant antibody (81798-2-RR, Clone:230306B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of NIH/3T3 using 81798-2-RR

  Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/mll) overnight. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of ATF4 (81798-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 81798-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81798-2-RR against Human ATF4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T
• Résultats positifs en IF/ICC: cellules HeLa traitées à la tunicamycine,
• Résultats positifs en FC (Intra): cellules HEK-293,
• Résultats positifs en ChIP-qPCR: Tunicamycin-NIH/3T3 (2ug/ml, overnight) NIH/3T3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81798-2-RR cible ATF4 dans les applications de WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATF4 Protéine recombinante Ag1279
• Nom complet: activating transcription factor 4 (tax-responsive enhancer element B67)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022088
• Symbole du gène: ATF4
• Identification du gène (NCBI): 468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.

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