Anticorps Monoclonal anti-ATF4

ATF4 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E4C5

N° de cat : CL488-60035

Synonymes

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB

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Galerie de données de validation

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  IF Staining of HeLa using CL488-60035

  Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using CL488-60035 (ATF4 antibody) at dilution of 1:50.

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  FC experiment of HeLa using CL488-60035

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATF4 (CL488-60035, Clone:3E4C5) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60035 cible ATF4 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATF4 Protéine recombinante Ag1279
• Nom complet: activating transcription factor 4 (tax-responsive enhancer element B67)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022088
• Symbole du gène: ATF4
• Identification du gène (NCBI): 468
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. This antibody is a mouse monoclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kd ATF4 protein and its phosphorylated forms (50 kd). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C