Anticorps Polyclonal de lapin anti-ATF6

• Phare
• Validé par KD/KO

ATF6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

216

Avis (1)

N° de cat : 24169-1-AP

Synonymes

ATF6, ATF6 alpha, ATF6A

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Galerie de données de validation

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  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24169-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24169-1-AP

  WB result of ATF6 antibody (24169-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 24169-1-AP

  WB result of ATF6 antibody (24169-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells.

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  WB analysis of HeLa using 24169-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24169-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24169-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24169-1-AP

  IP Result of anti-ATF6 (IP:24169-1-AP, 4ug; Detection:24169-1-AP 1:1500) with HeLa cells lysate 2800ug.

• 

  IP experiment of HeLa using 24169-1-AP

  IP result of anti-ATF6(IP:24169-1-AP, 4ug; Detection:24169-1-AP 1:1500) with HeLa cells lysate 680 ug.

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  IHC staining of human cervical cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast hyperplasia using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24169-1-AP (ATF6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24169-1-AP (ATF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24169-1-AP

  Immunofluorescent analysis of HeLa cells using 24169-1-AP (ATF6 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 24169-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (24169-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du pancréas humain, tissu d'hyperplasie mammaire humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 201 publications below
• IHC: See 13 publications below
• IF: See 15 publications below
• IP: See 2 publications below

Informations sur le produit

24169-1-AP cible ATF6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATF6 Protéine recombinante Ag21456
• Nom complet: activating transcription factor 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 60 kDa, 90-100 kDa
• Numéro d’acquisition GenBank: BC014969
• Symbole du gène: ATF6
• Identification du gène (NCBI): 22926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
human	IP	J Clin Invest; Ubiquitination of ATF6 by disease-associated RNF186 promotes the innate receptor-induced unfolded protein response.; Authors - Kishu Ranjan
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; Inhibition of the unfolded protein response reduces arrhythmia risk after myocardial infarction.; Authors - Man Liu
human	WB	Circ Res; ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart.; Authors - Jung-Kang Jin
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Richard (Verified Customer) (11-12-2019)

The antibody works well and gives two clean bands for cleaved and uncleaved ATF6 by western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeLa, HEK293T

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