Anticorps Monoclonal anti-ATF6

• Phare
• Validé par KD/KO

ATF6 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

21

CloneNo.

3B7E4

N° de cat : 66563-1-Ig

Synonymes

ATF6, ATF6 alpha, ATF6A

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Galerie de données de validation

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  WB analysis using 66563-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66563-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66563-1-Ig

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells.

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  WB analysis of HeLa using 66563-1-Ig

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells.

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  WB analysis of 4T1 using 66563-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66563-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.

• 

  WB analysis using 66563-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.

• 

  WB analysis of HSC-T6 using 66563-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.

• 

  WB analysis of NIH/3T3 using 66563-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.

• 

  WB analysis of RAW 264.7 using 66563-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.

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  IHC staining of human cervical cancer using 66563-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66563-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66563-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66563-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66563-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66563-1-Ig (ATF6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66563-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (66563-1-Ig, Clone:3B7E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IF: See 2 publications below

Informations sur le produit

66563-1-Ig cible ATF6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATF6 Protéine recombinante Ag21456
• Nom complet: activating transcription factor 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC014969
• Symbole du gène: ATF6
• Identification du gène (NCBI): 22926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Publications

Species	Application	Title
human	WB	Protein Cell; POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.; Authors - Jian Xiao
mouse	WB	J Cell Biol; Antigen-derived peptides engage the ER stress sensor IRE1α to curb dendritic cell cross-presentation.; Authors - Ofer Guttman
human	WB	Cell Rep; CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance; Authors - Masaki Takasugi
human	WB	Elife; Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers.; Authors - Vladislav Belyy
human	WB	Mol Oncol; The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma; Authors - George Luo
human	WB,IF	J Inflamm (Lond); Soluble epoxide hydrolase deficiency attenuates airway inflammation in COPD via IRE1α/JNK/AP-1 signaling pathway; Authors - Yue Yu