Anticorps Monoclonal anti-ATF6

ATF6 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3B7E4

N° de cat : CL594-66563

Synonymes

ATF6, ATF6 alpha, ATF6A



Applications testées

Résultats positifs en IFcellules HepG2,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66563 cible ATF6 dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène ATF6 Protéine recombinante Ag21456
Nom complet activating transcription factor 6
Masse moléculaire calculée 75 kDa
Poids moléculaire observé 90-100 kDa
Numéro d’acquisition GenBankBC014969
Symbole du gène ATF6
Identification du gène (NCBI) 22926
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Protocole

Product Specific Protocols
IF protocol for CL594 ATF6 antibody CL594-66563Download protocol
Standard Protocols
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