Anticorps Polyclonal de lapin anti-ATF6B

• Phare
• Validé par KD/KO

ATF6B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 15794-1-AP

Synonymes

CREB RP, cAMP-responsive element-binding protein-like 1, cAMP-dependent transcription factor ATF-6 beta, cAMP response element-binding protein-related protein, ATF6-beta

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Galerie de données de validation

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  WB analysis of Jurkat using 15794-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15794-1-AP (ATF6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15794-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15794-1-AP (ATF6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15794-1-AP

  IP result of anti-ATF6B (IP:15794-1-AP, 4ug; Detection:15794-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human brain using 15794-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15794-1-AP (ATF6B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15794-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15794-1-AP (ATF6B antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATF6B antibody (15794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu testiculaire humain
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

15794-1-AP cible ATF6B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATF6B Protéine recombinante Ag8502
• Nom complet: activating transcription factor 6 beta
• Masse moléculaire calculée: 703 aa, 77 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC008394
• Symbole du gène: ATF6B
• Identification du gène (NCBI): 1388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)

Publications

Species	Application	Title
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
human	WB	Int J Mol Sci; Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.; Authors - Francesca Potenza
mouse,human	WB,IHC,IF	Front Cell Dev Biol; miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress; Authors - Chikako Hayashi ; KD Validated
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
rat	WB	Front Cell Dev Biol; Mfn2 Regulates High Glucose-Induced MAMs Dysfunction and Apoptosis in Podocytes via PERK Pathway; Authors - Yun Cao
rat	WB	Arch Toxicol; Correction to: Protective effects of dioscin against alcohol‑induced liver injury.; Authors - Tingting Xu

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