Anticorps Polyclonal de lapin anti-ATG12

• Phare
• Validé par KD/KO

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11264-1-PBS

Synonymes

FBR93, Autophagy-related protein 12, APG12-like, APG12L, APG12 like

---

Galerie de données de validation

• 

  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 11264-1-AP (same clone as 11264-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 11264-1-AP (same clone as 11264-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATG12 antibody (11264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11264-1-PBS cible ATG12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG12 Protéine recombinante Ag1791
• Nom complet: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: BC012266
• Symbole du gène: ATG12
• Identification du gène (NCBI): 9140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

{{ptg:RelatedPrimaryAntibodies}}