Anticorps Recombinant de lapin anti-ATG12

ATG12 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242483F1

N° de cat : 84935-6-PBS

Synonymes

242483F1, APG12, APG12 like, APG12L, APG12-like

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Galerie de données de validation

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  WB analysis using 84935-6-RR (same clone as 84935-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84935-6-RR (ATG12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84935-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84935-6-RR against Human ATG12 were performed. The affinity constant is 0.637 nM.

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Informations sur le produit

84935-6-PBS cible ATG12 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATG12 Protéine recombinante Ag1791
• Nom complet: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC012266
• Symbole du gène: ATG12
• Identification du gène (NCBI): 9140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

{{ptg:RelatedPrimaryAntibodies}}