Anticorps Recombinant de lapin anti-ATG13

• Phare
• Validé par KD/KO

ATG13 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G14

N° de cat : 82718-3-PBS

Synonymes

KIAA0652, 3G14, Autophagy related protein 13, Autophagy-related protein 13

---

Galerie de données de validation

• 

  WB analysis using 82718-3-RR (same clone as 82718-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 82718-3-RR (same clone as 82718-3-PBS)

  WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82718-3-RR (same clone as 82718-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82718-3-RR (ATG13 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82718-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

82718-3-PBS cible ATG13 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATG13 Protéine recombinante Ag13090
• Nom complet: KIAA0652
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC001331
• Symbole du gène: ATG13
• Identification du gène (NCBI): 9776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

{{ptg:RelatedPrimaryAntibodies}}