Anticorps Monoclonal anti-ATG16L1

ATG16L1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5G7

N° de cat : 67943-1-PBS

Synonymes

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

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Galerie de données de validation

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  WB analysis using 67943-1-Ig (same clone as 67943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 67943-1-Ig (same clone as 67943-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67943-1-Ig (same clone as 67943-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67943-1-PBS cible ATG16L1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG16L1 Protéine recombinante Ag14881
• Nom complet: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: BC000061
• Symbole du gène: ATG16L1
• Identification du gène (NCBI): 55054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

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