Anticorps Polyclonal de lapin anti-ATG2A

• Phare
• Validé par KD/KO

ATG2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23226-1-PBS

Synonymes

KIAA0404, Autophagy-related protein 2 homolog A

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Galerie de données de validation

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  WB analysis using 23226-1-AP (same clone as 23226-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23226-1-AP (same clone as 23226-1-PBS)

  IP result of anti-ATG2A (IP:23226-1-AP, 4ug; Detection:23226-1-AP 1:4000) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 23226-1-AP (same clone as 23226-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATG2A antibody (23226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23226-1-PBS cible ATG2A dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG2A Protéine recombinante Ag19709
• Nom complet: ATG2 autophagy related 2 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 1938 aa, 213 kDa
• Poids moléculaire observé: 215 kDa
• Numéro d’acquisition GenBank: BC110650
• Symbole du gène: ATG2A
• Identification du gène (NCBI): 23130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.

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