Anticorps Monoclonal anti-ATG4B

• Phare
• Validé par KD/KO

ATG4B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5A7

N° de cat : 67727-1-PBS

Synonymes

Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B

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Galerie de données de validation

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  WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 67727-1-Ig (same clone as 67727-1-PBS)

  WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67727-1-Ig (same clone as 67727-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67727-1-PBS cible ATG4B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG4B Protéine recombinante Ag30510
• Nom complet: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000719
• Symbole du gène: ATG4B
• Identification du gène (NCBI): 23192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.

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