Anticorps Recombinant de lapin anti-ATG4D

ATG4D Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241035G8

N° de cat : 83951-4-PBS

Synonymes

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

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Galerie de données de validation

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  WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83951-4-PBS cible ATG4D dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATG4D Protéine recombinante Ag10177
• Nom complet: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC068992
• Symbole du gène: ATG4D
• Identification du gène (NCBI): 84971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

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