Anticorps Monoclonal anti-ATG5

• Phare
• Validé par KD/KO

ATG5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D3B8

N° de cat : 66744-1-PBS

Synonymes

2D3B8, APG5, APG5 LIKE, APG5L, APG5-like

---

Galerie de données de validation

• 

  WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66744-1-Ig (same clone as 66744-1-PBS)

  WB result of ATG5 antibody (66744-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66744-1-PBS cible ATG5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG5 Protéine recombinante Ag16685
• Nom complet: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 50-55 and 40-45 kDa
• Numéro d’acquisition GenBank: BC002699
• Symbole du gène: ATG5
• Identification du gène (NCBI): 9474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)

{{ptg:RelatedPrimaryAntibodies}}