Anticorps Recombinant de lapin anti-ATG5

• Phare
• Validé par KD/KO

ATG5 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5M5

N° de cat : 81803-1-PBS

Synonymes

Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5

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Galerie de données de validation

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  WB analysis using 81803-1-RR (same clone as 81803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

  WB result of ATG5 antibody (81803-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81803-1-RR (same clone as 81803-1-PBS)

  IP result of anti-ATG5 (IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 81803-1-RR (same clone as 81803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81803-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81803-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81803-1-RR against Human ATG5 were performed. The affinity constant is 42.4 nM.

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Informations sur le produit

81803-1-PBS cible ATG5 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATG5 Protéine recombinante Ag0214
• Nom complet: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC002699
• Symbole du gène: ATG5
• Identification du gène (NCBI): 9474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.

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