Anticorps Monoclonal anti-ATG7

• Phare
• Validé par KD/KO

ATG7 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B5A10

N° de cat : 67341-1-PBS

Synonymes

Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L, APG7 LIKE

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Galerie de données de validation

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  WB analysis using 67341-1-Ig (same clone as 67341-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis using 67341-1-Ig (same clone as 67341-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67341-1-Ig (same clone as 67341-1-PBS)

  WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  WB result of ATG7 antibody (67341-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67341-1-Ig (same clone as 67341-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 67341-1-Ig (same clone as 67341-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67341-1-PBS cible ATG7 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG7 Protéine recombinante Ag29172
• Nom complet: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 68-78 kDa
• Numéro d’acquisition GenBank: BC000091
• Symbole du gène: ATG7
• Identification du gène (NCBI): 10533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

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