Anticorps Recombinant de lapin anti-GABARAPL1

GABARAPL1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240533F5

N° de cat : 83817-1-PBS

Synonymes

240533F5, APG8 LIKE, APG8L, ATG8, ATG8L

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Galerie de données de validation

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  WB analysis using 83817-1-RR (same clone as 83817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83817-1-RR (ATG8L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83817-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83817-1-RR (same clone as 83817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83817-1-RR (GABARAPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83817-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83817-1-RR (same clone as 83817-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83817-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83817-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83817-1-RR against Human GABARAPL1 were performed. The affinity constant is 12.1 nM.

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Informations sur le produit

83817-1-PBS cible GABARAPL1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GABARAPL1 Protéine recombinante Ag1473
• Nom complet: GABA(A) receptor-associated protein like 1
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC009309
• Symbole du gène: GABARAPL1
• Identification du gène (NCBI): 23710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.

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