Anticorps Polyclonal de lapin anti-ATGL

• Phare
• Validé par KD/KO

ATGL Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55190-1-PBS

Synonymes

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 55190-1-AP (same clone as 55190-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

  WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 55190-1-AP (same clone as 55190-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 55190-1-AP (same clone as 55190-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

  IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55190-1-PBS cible ATGL dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: patatin-like phospholipase domain containing 2
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: NM_020376
• Symbole du gène: ATGL
• Identification du gène (NCBI): 57104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

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